【摘要】 目的:研究白细胞介素-1β(Interleukin-1β,IL-1β)对人冠状动脉平滑肌细胞(human coronary artery smooth muscle cell,HCASMC)基质金属蛋白酶3(matrix metalloproteinase3,MMP-3)金属蛋白酶组织抑制因子1(tissue inhibitor of metallopro-teinase1,TI MP-1)分泌平衡的影响。方法:培养HCASMC,至第5~7代时,分别加入20μg.L-1和0μg.L-1的IL-1β(0μg.L-1组设为对照组),均分别孵育2h、4h、8h、24h、36h后,收集细胞培养液上清;采用IL-1β(0、5、20、40μg.L-1)刺激HCASMC6h后分别收集细胞和细胞培养液上清。采用ELISA法检测细胞培养液上清内MMP-3及TI MP-1的表达量。结果:IL-1β组MMP-3,TI MP-1的表达量在2h时开始增加,并随时间的延长而不断增加,MMP-3/TI MP-1在2h开始升高,8h达到顶峰,后缓慢下降;随IL-1β剂量增加,MMP-3和TI MP-1的表达量、MMP-3/TI MP-1均不断升高。结论:IL-1β可造成HCASMC分泌不稳定斑块标记物MMP-3、TI MP-1及MMP-3/TI MP-1的升高,说明炎症可能是急性冠状动脉综合征发生发展的机制之一。更多还原

【Abstract】 Objective:To observe the effect of IL-1β on secretions balance of MMP-3 and TIMP-1 in HCASMC.Medthods:Incubation of HCASMC with IL-1β(20μg·L-1 and 0 μg·L-1),respectively,collect culture media after 2 h,4 h,8 h,24 h and 36 h;stimulate HCASMC with IL-1β(0,5,20,40μg·L-1),respectively,collect culture media after 6 h.Measure the concentrations of MMP-3 and TIMP-1 in cultural fluid by ELLISA.Results:Incubation of HCASMC with 20μg·L-1 IL-1β results in a time-dependent increase of MMP-3 and TIMP-1’s secreations,and MMP-3/TIMP-1 is up-regulation at 2 hour,get to the peak at 8 hour,then begin to descent slowly.Incubation the HCASMC with different concentrations of IL-1β caused a dose-dependent increase in MMP-3 and TIMP-1’s secreations and a increase of MMP-3/TIMP-1.Conclusion::IL-1β can induces the HCASMAC’s production of MMP-3 and TIMP-1,also the MMP-3/TIMP-1,and thus has implicated that inflammation may paly an important role in the development of acute coronary syndroms.更多还原