【摘要】 目的:探讨骨髓间充质干细胞(MSCs)移植对心肌梗死大鼠脾脏淋巴细胞体外杀伤效应的影响。方法:复制大鼠急性心肌梗死(AMI)模型,采用心外膜注射方法移植MSCs或PBS液,另取正常组、假手术组作对照。分别于手术后第1周、第2周和第4周末提取脾脏淋巴细胞,并与乳鼠心肌细胞共培养,采用结晶紫法检测淋巴细胞的杀伤效应。结果:手术后第2和4周末,AMI加PBS组脾脏淋巴细胞杀伤效应分别为(32.31±7.23)%、(47.20±5.13)%,较正常组[(21.95±4.65)%,(22.13±5.08)%]和假手术组[(22.21±5.43)%,(24.21±5.40)%]明显增强(均P<0.05)。相对于AMI加PBS组,AMI加MSCs组第2、4周末在一定程度上抑制了淋巴细胞的杀伤效应,分别为(26.14±5.15)%、(34.28±6.13)%,与AMI加PBS组的同时间比较,均P<0.05。结论:MSCs移植可抑制心肌梗死大鼠脾脏淋巴细胞对心肌细胞的杀伤效应。更多还原

【Abstract】 Objective:To investigate whether mesenchymal stem cells(MSCs)could inhibit lymphocyte cytotoxic activity against cardiac myocytes after myocardial infarction(MI).Method:SD rats were subjected to MI by left coronary artery occlusion.MSCs or PBS was injected into MI rats from epicardium instantly.In addition,normal and sham-AMI rats were made as control groups.Splenocytes were abstracted at 1,2 and 4 weeks after MI and were cultivated with cardiac myocytes of neonate rats.Lymphocyte cytotoxic activity against cardiac myocytes was detected by crystal-violet staining assay.Result:After MI,cytotoxic activity of PBS groups at 2 and 4 weeks[(32.31±7.23)%,(47.20±5.13)%] was higher than normal groups[(21.95±4.65)%,(22.13±5.08)%] and sham-AMI groups([22.21±5.43]%,[24.21±5.40]%)Compared with PBS groups,MSCs inhibited the cytotoxic activity in some extent([26.14±5.15]%,[34.28±6.13]%).Conclusion:MSCs transplantation can inhibit the cytotoxic activity of lymphocyte from splenocytes in rats with MI.更多还原