【摘要】 目的:从GFP+-大鼠胚胎脊髓分离和培养神经干细胞(NSC),观察NSC的分化功能和Neuregulin-1的表达。方法:从孕16d的GFP+-大鼠胚胎脊髓中分离、培养神经干细胞,用免疫组织化学方法观察神经球的Neuregulin-1表达及鉴定分化的细胞类型。结果:从大鼠胚胎脊髓能分离、培养出NSC。神经球能表达Neuregin-1和Nestin,并能进一步分化为神经元、星形胶质细胞和少突胶质细胞。结论:从GFP+-大鼠胚胎脊髓能分离和培养出NSC,该NSC具有分化为用于治疗中枢神经疾病的多种神经细胞的潜能。更多还原

【Abstract】 Aim:To isolate and culture the neural stem cells(NSCs) from spinal cord of the GFP+-transgene embryonic rat,and to observe the multiple functions and Neuregulin-1 expression of NSCs. Methods:NSCs were isolated and cultured from spinal cord of the GFP+-embryonic rat(E16d) . The Neuregulin-1 expression of neurospheres and identification of cellular types differentiated from NSCs were determined by the method of immuno-histochemistry. Results:NSCs could be isolated and cultured from spinal cord of the GFP+-embryonic rat. The neurospheres could express Neuregulin-1 and Nestin,and it could further differentiate into neurons,astrocytes and olygodenrocytes. Conclusion:NSCs could be isolated and cultured from spinal cord of the GFP+-embryonic rat. The NSCs have multiple functions of which differentiate into various neurocytes used in the treatment of CNS diseases.更多还原