【摘要】 目的探讨凋亡调控蛋白Fas/FasL、Bcl-2/Bax在Graves病(GD)和桥本氏病(HT)中的表达及意义。方法取外科手术切取的GD及HT病人甲状腺组织标本,颈部手术时取正常甲状腺组织作为对照;采用免疫组织化学方法检测甲状腺标本Fas/FasL、Bcl-2/Bax的表达和分布状况。结果(1)Fas/FasL在GD和HT甲状腺滤泡上皮细胞表达均较正常对照组增高(P<0.01)。(2)在GD甲状腺滤泡上皮细胞Bcl-2及Bax表达明显高于HT组及正常对照组,以Bcl-2表达强度显著高于同组Bax(P<0.01),而在HT,Bcl-2表达强度与正常对照组比较差异无统计学意义。(3)GD与HT相比较,GD甲状腺滤泡细胞及浸润淋巴细胞Fas,Bcl-2抗原表达均强于HT,但其浸润淋巴细胞表达强度明显低于同组甲状腺滤泡细胞(均P<0.05)。结论Fas/FasL高表达和Bcl-2的低表达可能引起HT甲状腺滤泡上皮细胞的凋亡。在GD,Fas、Bax诱导细胞凋亡的作用可能被高表达Bcl-2对抗,从而致甲状腺体积增大、功能亢进。此外,Bcl-2与Bax表达强度的比值对于凋亡的调控有重要影响作用。更多还原

【Abstract】 Objectives To study the expression of Fas/FasL,Bcl-2/Bax in Graves disease(GD) and Hashimoto’s thyroiditis(HT) and to investigate the relationship between apoptosis and autoimmune thyroid disease(AITD).Methods Thyroid tissues were obtained by surgical intervention,immuunohisttochemical methods were used to examine the expression of Fas/FasL,Bax、Bcl-2 antigen on thyrocytes and lymphocytes.Results(1)The Fas/FasL in the thyroid follicular cells from patients with GD and HT were higher than those from normal controls(P<0.01).(2)The Bcl-2/ Bax in the thyroid follicular cells from patients with GD were higher than those from normal controls and HT(P<0.01),and the expression of Bcl-2 was also stronger than Bax.The positive rates of Bcl-2 expression in HT there was no significant difference with normal contrals.(3)The expression of Fas,Bcl-2 on thyoid follicular cells and infiltrated lymphocytes of GD was stronger than that of HT.Conclusion(1)The increased expression of Fas/FasL and reduced expression of anti-apoptosis regulated protein Bcl-2 seems to be related to induce apoptosis with thyroid tissues in HT.But in GD,the effect of apoptosis induced by Fas,Bax might be overcome by Bcl-2.Besides,the ratio of Bcl-2/Bax affects cell apoptosis.更多还原