【摘要】 目的检测系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)程序性死亡配体-1(PD-L1)的表达水平,探讨其在系统性红斑狼疮发病过程中的作用。方法密度梯度离心法分离系统性红斑狼疮患者和正常人PBMC,采用半定量RT-PCR检测PBMC的PD-L1mRNA表达。结果系统性红斑狼疮患者活动期组和稳定期组的PD-L1mRNA表达与对照组比较明显增高,差异有显著性(P均<0.05);但PD-L1mRNA表达在系统性红斑狼疮活动期组与稳定期组之间比较无显著性差异(P>0.05)。结论系统性红斑狼疮患者外周血单个核细胞PD-L1mRNA表达水平增加,程序性死亡配体-1可能在系统性红斑狼疮的发病机制中有一定的作用。更多还原

【Abstract】 Objective To investigate the expression of programmed death-ligand 1(PD-L1) on peripheral blood mononuclear cells(PBMCs) in patients with systemic lupus erythematosus(SLE).Methonds The PBMCs of SLE patients and controls were isolated by Ficoll density gradient medium and mRNA expression of PD-L1 was detected by half-quantitative RT-PCR.Results The expression level of PD-L1mRNA in active SLE group and inactive SLE group was found to be higher than that of the normal control group(P<0.05,P<0.05,respectively).But there was no statistically significant difference between active SLE group and inactive SLE group(P>0.05).Conclusion The expression of PD-L1mRNA on PBMCs in patients with SLE was significantly increased.It can be suggested that the PD-L1 might play some role in the pathogenesis of SLE.[更多还原