【摘要】 目的通过检测支气管肺泡灌洗液(BALF)中端粒酶的活性,探讨BALF端粒酶活性在肺外周孤立性结节诊断中的应用价值。方法采用TRAPeze-ELISA法,检测113例经螺旋CT扫描发现的肺外周孤立性结节患者支气管镜下取得的BALF中脱落细胞端粒酶活性,并将检测结果与细胞学等结果相比较。结果54例肺癌组中端粒酶活性的阳性率为68.5%(37/54),而59例肺良性疾病组的阳性率为6.8%(4/59),肺癌组检出的敏感性为68.5%,特异性为93.2%。结论BALF中端粒酶活性测定有利于肺外周孤立性结节的定性诊断,为其中肺癌的早期诊断提供依据,同时也有利于肺癌与肺部良性疾病的鉴别诊断。更多还原

【Abstract】 Objective By testing the activity of telomerase exist in Bronchial Alveolar Lavage Fluid(BALF),research the application value of applying test of activity of BALF telomerase to diagnosing the isolated nodules at outside part of lung.Method Using TRAPeze-ELISA method,test samples of telomerase in BALF get by using bronchoscope from 113 patients who were diagnosed of isolated nodules at outside part of lung by spiral CT,and compare the test result to those result from Histopathology and Cytology.Result The positive rate of telomerase activity of 54 instances lung cancers is 68.5%(37/54),while the positive rate of 59 instances of benign tumors patient group is 6.8%(4/59),means the positive rate of lung cancer group is 68.5%,specific case is 93.2%.Conclusion The test of telomerase activity is useful for qualitative diagnosis of isolated nodules at outside part of lung,it will provide useful evidence for lung cancer early diagnosis;it also helps to identify lung cancer from benign lung disease.更多还原