【摘要】 目的:探讨普鲁卡因(procaine)对人鼻咽癌(NPC)细胞CNE-2Z的影响。方法:体外培养的NPC细胞CNE-2Z分别加入不同浓度的普鲁卡因,并作用不同的时间。应用四甲基偶氮唑盐[3-(4,5-Dimethyl-thiaoly)-2,5-diphenyl-2H tetrazolium bromide,MTT]法和流式细胞仪检测观察普鲁卡因对NPC细胞CNE-2Z的影响,同时用RT-PCR分析p16和RASSF1A mRNA的表达情况。结果:普鲁卡因能显著抑制CNE-2Z细胞增殖,该作用呈剂量-效应和时间依赖关系。普鲁卡因使细胞周期阻滞于G1期,并能上调CNE-2Z细胞RASSF1A mRNA的表达,但对p16 mRNA表达无明显影响。结论:普鲁卡因对CNE-2Z细胞有增殖抑制作用,它能上调CNE-2Z细胞RASSF1A mRNA的表达,推测这可能是普鲁卡因抑制CNE-2Z细胞增殖的重要分子机制之一。更多还原

【Abstract】 Objective:To study the effects of procaine on human nasopharyngeal carcinoma cell strain CNE-2Z.Method:CNE-2Z cells-cultured in vitro-were exposed to different concentration procaine and different time.Its growth characteristic were studied by using MTT assay and flow cytometry.The expresstion of p16 and RASSF1A mRNA were analyzed by RT-PCR.Result:Procaine could inhibit the proliferation of CNE-2Z cells and the effects were in doso-and time-dependent manners.Procaine also arrested CNE-2Z cells in G1 phase of cell cycle.The expression of RASSF1A mRNA was up-regulated in CNE-2Z cells treated with procaine,but no obvious change was found in the expression of p16 mRNA.Conclusion:Procaine could inhibit cell growth and up-regulate the expression of RASSF1A mRNA,which may play an important role in CNE-2Z cells’ proliferation inhibition induced by procaine.更多还原