【摘要】 目的探讨慢性应激致高血压过程中肾上腺髓质素特异性受体组件降钙素受体样受体、受体活性修饰蛋白2和受体活性修饰蛋白3在下丘脑的动态变化。方法将48只大鼠分为对照组(18只)和应激组(30只),应激组给予噪声加足底电击刺激,分别在刺激开始后的第1、5、10和第15d以及停止刺激后的第5和第10d处死动物,分离下丘脑,抽提总RNA,用逆转录聚合酶链式反应检测降钙素受体样受体、受体活性修饰蛋白2和受体活性修饰蛋白3的基因表达变化。结果与对照组相比较,应激组动物降钙素受体样受体基因表达10d内逐渐上调(1.236±0.040比0.941±0.041,P<0.01),而后表达下调,在应激第15d明显低于于对照组(0.860±0.041比0.938±0.042,P<0.05)。受体活性修饰蛋白2mRNA表达在应激第1和第5d显著升高(0.975±0.023比0.880±0.035和1.160±0.045比0.897±0.036,P<0.05或P<0.01),在第5d达到高峰,而后开始下调,在应激第15d和应激结束后5d低于对照组(0.670±0.021比0.897±0.041,0.719±0.039比0.910±0.032,P<0.01)。受体活性修饰蛋白3mRNA表达在应激第1d显著升高(1.197±0.053比0.870±0.039,P<0.01),而后开始逐渐下调,在应激第10、15d后开始明显低于对照组的表达水平(P<0.05或P<0.01)。应激结束后10d受体活性修饰蛋白3mRNA表达水平仍低于对照组,但无统计学差异。结论慢性应激所致血压升高变化过程中伴随下丘脑中肾上腺髓质素特异性受体组件mRNA表达的不同时段特异性改变,提示其可能参与应激致高血压过程。更多还原

【Abstract】 Aim To investigate the dynamic changes of adrenomedullin specific receptors components,calcitonin receptor-like receptor(CRLR),receptor activity modifying proteins 2(RAMP2) and RAMP3 mRNA expression in the stress-induced-hypertensive rats.Methods Distribute 48 rats to control group with 18 of them and stress group with 30 of the total randomly.Noise with electric foot shock upon the stress group.Execute all the rats and separate their hypothalamus to isolate the total RNA individually at stress 1,5,10 and 15 d and poststress 5 and 10 d.Determine the changes of the adrenomedullin,CRLR and RAMP mRNA expression of by reverse transcription-polymerase chain reaction(RT-PCR).Results Compared with control group at the same time point,CRLR mRNA expression in hypothalamus upregulated within foot-shock and sound stress 10 d(1.236±0.040 vs 0.941±0.041,P<0.01),then downregulated to a lower level at stress 15 d(0.860±0.041 vs 0.938±0.042,P<0.05);RAMP2 mRNA expression in hypothalamus upregulated to the highest lever at stress 5 d(1.160±0.045 vs 0.897±0.036,P<0.01),then downregulated to a lower lever at stress 15 d and poststress 5 d(0.670±0.021 vs 0.897±0.041,0.719±0.039 vs 0.910±0.032,P<0.01);However RAMP3 mRNA expression upregulated to the highest lever at stress 1 d(1.197±0.053 vs 0.870±0.039,P<0.01),then downregulated to a lower lever at 10,15 d and poststress 5 d(P<0.05 or P<0.01).Even at poststress 10 d,the level of RAMP3 expression still lower than control group at the same time point,but no statistic significance.Conclusion According to the results described above,at the gene level,the expression of ADM specific receptor components CRLR,RAMP2 and RAMP3 mRNA had changed to some degree in the process of chronic stress-induced-hypertension,indicating that ADM specific receptors maybe involve in the stress-induced-hypertension.更多还原