【摘要】 目的以拉力为参数建立不同程度的腰骶神经根牵拉损伤动物模型。方法将40只中国大白兔随机分为对照组、轻度牵拉组、中度牵拉组和重度牵拉组。全椎板切除显露双侧S1神经根,用测力神经根拉钩分别以不同的拉力造成神经根的牵拉性损伤。行体感诱发电位(somatosensory evoked potential,SEP)监测和神经功能评分,神经根和骶髓做组织病理学研究。结果轻度牵拉组SEP潜伏期比对照组稍延长(P>0.05),神经根和骶髓结构正常,Hashi-zume行为学测试正常;中度牵拉组SEP潜伏期明显延长(P<0.05),去除牵拉后潜伏期接近正常(P>0.05),神经根结构轻度异常,骶髓结构正常,Hashizume行为学测试轻度异常;重度牵拉组SEP潜伏期明显延长(P<0.01),去除牵拉后潜伏期稍缩短,和对照组的差异有统计学意义(P<0.05),神经根结构明显异常,骶髓轻度异常,Hashizume行为学测试异常。所有实验动物改良Tarlov运动功能评分均正常。结论以拉力为参数可以稳定地建立不同程度的腰骶神经根牵拉性损伤动物模型。更多还原

【Abstract】 ObjectiveTo establish an animal model of graded tractive lumbar-sacral nerve root injury in rabbits. MethodsForty rabbits were randomly divided into 4 groups: control group without traction, light traction group, middle traction group and serious traction group. The models were induced by dragging the first left sacral nerve root in different degrees of traction with a special retractor after exposing the spinal cord entirely via bilateral laminectomy, then neurophysiologic evaluation were performed and tissue samples were harvested for histological analyses. ResultsThe neural electrophysiological changes, behavioral functions and histological changes were correlated with that of tracion. The larger of the traction, the longer of the latency of SEP P1 wave and the more serve of the histological change and Hashizume behavior response. The light traction group showed no significant difference compared to the control group throughout the experimental period. For the middle traction group, the latency of SEP P1 wave were significantly longer than the control nerve roots during the traction, but not significantly after the traction. There was a statistically significant longer in the latency of SEP P1 wave and a higher pain response in the serve traction group compared with the control group after 24 h and after 7 d. The nerve roots displayed tissue swelling, nerve fiber damage, hyperemia and inflammation. Only slight damage of sacral cord could be found in the serve traction group. ConclusionThis animal model of tractive lumbar-sacral nerve root injury based on different degrees of traction. It has stable and repetitive characters for research on basic pathophysiologic mechanisms and on the effects of various interventions on nerve root injury development.更多还原