【摘要】 针对以苯甲醛、氯仿为原料合成苯甘氨酸的反应过程,建立了高效液相色谱法测定苯甘氨酸含量的方法,考察了流动相组成与流量对分离效果的影响,得出了适宜的分析条件。以10%甲醇+90%磷酸二氢钠溶液(20 mmol/L)为流动相,在其流量1 mL/min、柱温为室温和检测波长260 nm的条件下,苯甘氨酸质量浓度在25～400 mg/L范围内,质量浓度与峰面积呈较好的线性关系,系统精密度指标RSD值为0.13%。更多还原

【Abstract】 The high performance liquid chromatography(HPLC)method for the analysis of phenylg- lycine in the synthesis of phenylglycine from benzaldehyde and chloroform was established.The influ- ences of the composition and the flow-rate of the mobile phase on the separation were examined.The optimum chromatography conditions were found as follows.V(methanol):V[monosodium phosphate (20 mmol/L)]=1:9 as the mobile phase,flow-rate of mobile phase 1 mL/min,detection wavelength 260 nm.The standard curve for phenylglycine was linear over the range of 25-400 mg/L.The RSD value for the accuracy of the system was 0.13%.更多还原