【摘要】 当龙牙百合鳞茎在58℃下处理10~20 min,再在40℃下处理72 h;茎尖大小为0.2~0.4 mm时的诱导成活率达60%~66.7%。继代增殖培养基为MS+1.0 mg/L BA+0.1 mg/L NAA时,其增殖系数是3.36。培养基中4.5%~5.5%的食用糖和60 d的培养时间利于鳞茎数量和质量的增加。移栽后的小鳞茎培育8个月可达鲜重22 g/个以上的脱毒种球。更多还原

【Abstract】 Longya lilium bullets were first treated at 58 ℃ for 10~20 minutes then at 38 ℃ for 72 h.The survival ratio with shoot-tip of 0.2~0.4 mm was 60.0%~66.7%.The reprogactiving coefficient of bulb was 3.36 when a medium for continual culture was MS+1.0 mg/L BA+0.1 mg/L NAA.The quantity and quality of bullet would be increased with a media added with 4.5%~5.5% sugar and cultured for 60 days.Each transplanted bulb could become virus-free bullet which weighed more than 22 g after 8 months’ cultivation.更多还原