【摘要】 SLC6A14基因是影响猪脂肪沉积的候选基因,本研究利用白色杜洛克×二花脸资源家系祖代的17头二花脸母猪和2头白色杜洛克公猪的池DNA,通过直接测序法在猪SLC6A14基因第10外显子1483 bp处检测到1个G→A错义突变,导致相应编码的氨基酸由缬氨酸转换成异亮氨酸。采用引物创造性酶切法,通过HpyCH4 IVPCR-RFLP判型技术,检测了白色杜洛克×二花脸资源家系3代623个个体在此错义突变位点的多态性,并开展了与脂肪和生长性状的相关性分析。结果表明:该突变位点与腹脂重和46日龄个体重存在显著的相关性。更多还原

【Abstract】 SLC6A14 was considered as a candidate gene for fat deposition.A G→A missense mutation(SLC6A14 c.1483>A) was identified at position 1483 in exon 10 of the porcine SLC6A14 gene by directly sequencing DNA pools from 19 founder animals(two White Duroc boars and 17 Erhualian sows) of a White Duroc × Erhualian intercross.This mutation leads to an exchange of valline to isoleucine at codon 461.A total of 623 animals from the White Duroc × Erhualian intercross were genotyped for the SLC6A14 c.1483>A polymorphism using a PCR primer induced mutagenesis method,and associations of this polymorphism with growth and fatness traits were performed in this population.The results showed that the SLC6A14 c.1483>A polymorphism was significantly associated with abdomen fat weight and body weight at 46 d of age.更多还原