【摘要】 根据猪圆环病毒2型GD株及已发表的PCV2的全基因组序列,设计1对PCV2型特异性引物,对可疑病料进行PCR扩增。将扩增产物连接到pMD 18-T载体上并克隆到大肠杆菌DH5α中,提取质粒进行套式PCR、酶切、测序鉴定,结果扩增出了PCV2的目的片段。将测序结果与GenBank收录的PCV2序列进行比较,发现同源性均在90%以上。用该PCR方法对287个猪场的1 560份样品进行了检测,其中983份为PCV2阳性,阳性率为63%。更多还原

【Abstract】 According to the published genomo of porcine circovirus type 1 and type 2(PCV1,PCV2),two pairs of special primers were designed to detect PCV2 in samples from some farms by nested PCR assay. The PCR product was extracted and subcloned into pMD 18-T vector and then cloned into E.coli DH5α.The recombination plasmid was identified by digestion with different enzyme restrictions and se- quencing analysis.The homology of nucleotide sequence between the PCR product and that of other PCV2 strains was more than 90%.Using this PCR method,1 560 samples from 287 pig farms have been detect- ed for PCV2,from which 983 samples got the positive results for PCV2,suggesting that PCV2 infection is very popular in China.更多还原