【摘要】 以逆境性相关转录因子DREB2A为目的基因、除草剂抗性的bar基因为标记,构建了植物表达载体pCD2A,并通过农杆菌介导法以无菌苗子叶为外植体转化公农1号苜蓿。经过共培养、抑菌和筛选培养,在含有2 mg/L Basta的培养基获得71株抗性再生植株。用1 mg/L的Basta对抗性植株叶片进行进一步筛选,并经PCR检测,获得11株阳性植株,表明DREB2A已整合到植株的基因组中。更多还原

【Abstract】 Using the stress related transcriptional factor gene,DREB2A,as the target gene,and the herbicide tolerance resistant gene,bar,as the selective marker,a plant expression vector,pCD2A,was constructed.Transfor- mation was performed via the Agrobacterium-mediated method on the alfalfa cultivar‘Gongnong No.1’using the cotyledons for explants.After co-culture,bacteria restraining and selective culture,71 resistant regenerated plants were obtained on the medium with 2mg/L Basta.A further selection on leaves of the resistant plants,11 positive plants were obtained by PCR,which indicated that the gene DREB2A has inserted into the genome of the plant.更多还原