【摘要】 目的研究血管紧张素转换酶(ACE)基因2350G→A单核苷酸多态性与心肌梗死(MI)的相关性。方法选择84例MI患者及年龄、性别相匹配的135例正常对照组,聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测ACE基因型。比较两组基因型及等位基因频率分布以及各基因型对临床参数的影响。结果MI组AA基因型频率(26.19%)明显高于对照组(14.81%)(P=0.0376),而GG基因型频率(38.10%)明显低于对照组(56.30%)(P=0.0088);与对照组相比,MI组A等位基因频率(44.05%)明显升高,G等位基因频率(55.95%)明显降低(P=0.016);无论是MI组还是对照组均未发现ACE基因型对血脂、载脂蛋白、高敏C反应蛋白以及可溶性CD40配体水平有影响。结论ACE基因2350G→A单核苷酸多态性与心肌梗死相关,AA基因型及A等位基因可能是心肌梗死的遗传易患因子,并且这种遗传相关性与血脂及急性冠脉综合征标志物水平无关。更多还原

【Abstract】 Objective To study the association of unique nueleotide polymorphism(UNP)of 2350G→A in the angiotensin converting enzyme(ACE)gene with myocardial infarction(MI)susceptibility in Han nationality. Method ACE genotypes of 84 patients of MI and 135 healthy subjects as control were detected by PCR-RFLP. ACE genotypes and alleles frequencies were compared between two groups,and the impacts of ACE genotypes on plasma levels of lipids,apolipoproteins,as well as high sensitive C-reacting protein(hs-CRP),and soluble CD40 ligand(sCD40L)were also evaluated.Results The frequencies of AA genotype in MI group(26.19%) were higher than those in control subjects(14.81%)(P=0.0376),while GG genotype in MI group (38.10%)were lower than those in control subjects(56.30%)(P=0.0088).The patients with AA genotype had twice risk for MI in comparison with GG genotype(OR=2.04,95%CI=1.04～4.00).There were no significant differences among three genotypes on plasma levels of lipids,apolipoproteins,hs-CRP and sCD40L for either MI group or healthy control group(P>0.05).Conclusions SNP of 2350G→A in ACE gene is associated with MI,AA genotype is probably a genetic marker of MI in Han nationality.更多还原