【摘要】 经RT-PCR和RACE合成了柳蚕卵黄原蛋白(Vg)cDNA,经序列分析其长度为5701个碱基,由一个ORF组成,编码了1 774个氨基酸。氨基酸序列中有2个保守的多聚丝氨酸区域。并推测氨基酸序列中有8个天冬酰胺连接的糖基化位点(N-linked glycosylation sites)。与樟蚕、天蚕、柞蚕、野桑蚕、家蚕、樗蚕、蓖麻蚕的VgcDNA序列比对后发现有很高的同源性,分别为83.1%、81.1%、81.0%、64.7%、64.6%、79.7%、79.2%,同其它昆虫Vg cDNA序列也具有很高的同源性。根据7种蚕(家蚕、天蚕、柞蚕、蓖麻蚕、樟蚕、野桑蚕、樗蚕)VgcDNA序列建立了系统发生树。更多还原

【Abstract】 The cDNA sequence of vitellogenin in Actias selene Hubner is obtained through RT-PCR to the total RNA and RACE amplification to 3’-end,which contains 5 701 bases,an opening reading frame which encodes 1 774 amino acids,composed of 8 N-linked glycosylation sites,2 polyserne regions,Homology of bases between Actias selene Hubner,Saturnia japonica(AB190809),Antheraea yamamai(AB055843),Antheraea pernyi(AB049631),Bombyx mandarina(AB055845),Bombyx mori(D13160),Samia cynthia pryeri(AB190810),Philosamia cynthia ricini(ab0555844).Homology identities are 83.1 %、81.1 %、81.0 %、64.7 %、64.6 %、79.7 %、79.2 % respectively.Homology identity is high compared with other insects.A phylogenetic tree was established based on the vitellogenin cDNA of seven species,including Antheraea pernyi,Antheraea yamamai,Bombyx mori,Philosamia cynthia ricini,Bombyx mandarina,Samia cynthia pryeri,Saturnia japonica.更多还原