【摘要】 目的探讨口服卡介菌(BCG)治疗Ⅰ型超敏反应的疗效和可能机制。方法以卵清蛋白(OA)诱导小鼠过敏性哮喘,通过口服途径给药,观察不同剂量BCG对哮喘症状的出现时间,严重程度的影响;ELISA方法和PCA方法检测特异性IgG、IgE抗体滴度;RT-PCR方法检测IL-1、2、10、12 mRNA表达的变化。结果BCG在0.5~2.0 mg/kg剂量范围内,能够减少哮喘小鼠单位时间内的搔鼻次数,推迟攻击后哮喘发作的时间,降低肺气道阻力,较少特异性IgE抗体的生成,调节细胞因子mRNA的表达。结论口服BCG对治疗过敏性哮喘有效,作用机制可能与特异性抗原口服免疫耐受不同。更多还原

【Abstract】 Objective To explore the effects and possible mechanisms of oral BCG in treating anaphylactic-type Ⅰ reaction.Methods Ovalbumin sensitized mice were treated with BCG from low-dose to high-dose by oral application and the emergence time,severity of asthma symptoms and the effects on antibody titre of specific IgE and IgG and the mRNA of IL-1,2,10,12 were recorded.Results Oral BCG range from 0.5 mg/kg to 2.0 mg/kg can reduce the frequency of nose scraping in allergic mice in a standard times and delay the emergence of asthma symptoms,reduce the air way resistance and the generation of the antibody titre of specific IgE,and regulate the mRNA expression of cytokins.Conclusion Oral BCG is effective in treatment of allergic asthmain mouse model.The mechanism of induced tolerance immune is different with the specific antigen and BCG.更多还原