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J-亚群禽白血病病毒中国广东野毒株的分离与鉴定
Isolation and Identification of Subgroup J Avian Leukosis Virus
【摘要】 通过接种鸡胚成纤维细胞、间接荧光抗体试验(IFA)和聚合酶链式反应(PCR)从疑似J-亚群白血病的病鸡中,分离鉴定出J-亚群白血病病毒。抗J-亚群白血病病毒gp85单克隆抗体JE9的IFA试验和PCR试验均证明病鸡被J-亚群白血病病毒感染。
【Abstract】 Subgroup JAvian Leukosis Virus was isolated and identified from a breeding farm with sus- pected sick chickens by inoculating the samples into embryo fibroblast,by indirect immunofluorescence as- say(IFA)and PCR amplification technology.The results of IFA with ALV-J specific monoclonal antibody JE9 and PCR showed that the suspected sick chickens had been infected by ALV-J.
- 【文献出处】 家畜生态学报 ,Acta Ecologiae Animalis Domastici , 编辑部邮箱 ,2007年05期
- 【分类号】S852.65
- 【被引频次】16
- 【下载频次】172