【摘要】 应用荧光光谱及紫外可见光谱的方法研究了2-氨基-5-苯亚甲基-4H-咪唑啉-4-酮衍生物(BPH5)与牛血清白蛋白(BSA)的相互作用.实验发现BPH5能强烈猝灭牛血清白蛋白的荧光强度,其荧光猝灭机理为动态猝灭.在此基础上计算了二者相互作用的结合常数、结合位点数及ΔH,ΔG,ΔS等热力学参数等.结果表明BPH5与BSA以1∶1结合,其反应主要是熵驱动的,相互作用力主要为疏水作用力.根据F rster无辐射能量转移理论计算了给体(BSA)与受体(BPH5)之间的结合距离.更多还原

【Abstract】 The interaction between(Z)-4-benzylidene-1-(4-chlorophenyl)-2-propylamino-1H-imidazol-5(4H)-one(BPH5) and bovine serum albumin(BSA) was studied by florescence spectroscopy and UV-vis absorption spectroscopy.The fluorescence intension of BSA could be quenched strongly by BPH5 in the experiment and the mechanism is a dynamic quenching procedure.The values of binding sites n and apparent binding constant Kb were measured by fluorescence quenching method.The thermodynamics parameter ΔH,ΔG,ΔS were calculated.The results indicated BPH5 and BSA were combined by 1∶1,the reaction was driven by entropy and the interaction force was mostly hydrophobic force.The distance r between donor(BSA) and acceptor(BPH5) was obtained according to Frster theory of non-radiation energy transfer.更多还原