【摘要】 目的体外诱导细胞因子诱导的杀伤细胞(CIK),并研究其生物学活性。方法从外周血分离单个核细胞(PBMC),经过细胞因子诱导、培养并扩增CIK细胞,以LAK细胞作比较,检测CIK的增殖能力,流式细胞仪检测CIK细胞表面标志CD3、CD56,MTT法检测对K562细胞系杀伤活性。结果CIK细胞第二周进入快速增殖期,到第21d扩增倍数超过120倍,CD3+、CD56+细胞扩增倍数达15倍以上;CIK对K562细胞的杀伤能力明显优于LAK细胞。结论CIK细胞是一种具有很强杀瘤活性的免疫活性细胞,具有临床应用前景。更多还原

【Abstract】 Objective To prepare cytokine-induced killer(CIK)in vitro,and study the biological activities of CIK.Methods Lymphocytes were isolated from peripheral blood and induced by cytokine.The proliferation of CIK was tested.The cells surface markers CD3 and CD56 of CIK were analyzed by FACS.The cytotoxic effect of CIK on K562 cells was measured by MTT.Results CIK cells quickly proliferated from the second week,and expanded more than 120-fold at the 21th day after induction.The CD+ 3and CD+ 56cells expanded more than 15 folds.The cytotoxic effect of CIK on K562 cells was obviously stronger than that of LAK cells.Conclusion CIK was a highly efficient cytotoxic cells against tumors,and had clinical application potentials.更多还原