【摘要】 目的探讨干扰素-γ(IFN-γ)对正常人精子顶体酶(acrosin)活力的影响及其作用机制。方法采用BAEE/ADH联合法测定精子顶体酶活力,用高效液相色谱(HPLC)测定一氧化氮(NO),用生化法测定超氧化物歧化酶(SOD)和一氧化氮合酶(NOS)。用定磷法测定Na+-K+-ATPase和Ca2+-ATPase活性。结果IFN-γ可降低精子顶体酶活力(P<0.05),明显降低精子中Na+-K+-ATPase、Ca2+-ATPase和SOD活性(P<0.001);但显著提高精子NOS活性和NO浓度(P<0.001)。结论IFN-γ可降低精子顶体酶活力,其机制可能与精子中Na+-K+-ATPase、Ca2+-AT-Pase、SOD和NOS活性以及NO浓度等因素有一定关系。更多还原

【Abstract】 Objective To investigate the effect of interferon-gamma(IFN-γ) on the activity of acrosin in human sperm and its mechanism.Methods The acrosin activity was detected by BAEE/ADH method.The nitric oxide(NO) concentration was tested by HPLC method and the activity of superoxide dismutase(SOD),nitric oxide synthase(NOS),Na+-K+-ATPase,Ca2+-ATPase were tested by the biochemical technique.Results IFN-γ decreased the activity of acrosin(P<0.05) and the activities of SOD,Na+-K+-ATPase,and Ca2+-ATPase(P<0.001),but increased the NOS activity and the NO concentration in human sperm.Conclusion IFN-γ has some inhibitory effect on sperm acrosin activity,which could be attribute to their influence on activities of Na+-K+-ATPase,Ca2+-ATPase SOD,NOS,the NO concentration and so on.更多还原