【摘要】 合成了两种新型三齿多吡啶钴(Ⅱ)和钌(Ⅱ)的混配配合物[Co(TolylTPy)(H2Bzimpy)]Cl2[TolylTPy=4’-对甲基苯基-2,2’:6’,2’’-三联吡啶,H2Bzimpy=2,6-二(苯并咪唑-2)吡啶](A)和Ru(TolylTPy)(Bzimpy)(B).用元素分析,IR,1HNMR等对它们进行了表征,测定了配合物B的晶体结构,用电子吸收光谱、荧光光谱等研究了配合物与小牛胸腺DNA(CTDNA)的相互作用及其对pBR322DNA的断裂作用.结果表明,配合物A和B与CTDNA的作用属静电结合,凝胶电泳实验说明配合物A在310nm光辐射15min,可使超螺旋pBR322DNA断裂为开环缺口型和线型DNA.更多还原

【Abstract】 Two novel tridentate polypyridyl mixed-ligand complexes of cobalt(Ⅱ) and ruthenium(Ⅱ),Co(TolylTPy)(H2Bzimpy)Cl2 TolylTPy=4’-p-tolyl-2,2’:6’,2’’-terpyridine,H2Bzimpy=2,6-bis(benzimi-dazol-2-yl)pyridine (A) and Ru(TolylTPy)(Bzimpy) (B) were synthesized and characterized by elemental analysis,IR and 1H NMR. The crystal structure of complex B was determined. The interactions between the complexes and calf thymus DNA have been investigated by absorption and fluorescence spectroscopies. The photoactivated cleavage of pBR322 DNA by the complexes was studied. The spectrophotometric studies suggested that both complexes A and B bind to DNA by electrostatic interactions. The agarose gel electro-phoresis showed that complex A converted supercoiled pBR322 DNA to nicked and linear DNA when it was irradiated at 310 nm for 15 min.更多还原