【摘要】 目的:利用重组复制缺陷型腺病毒AdIL-10将外源IL-10基因导入肝星状细胞并观察其对肝星状细胞Ⅰ、Ⅲ胶原分泌的影响。方法:用传至1~2代的大鼠肝星状细胞以2×105/孔接种于24孔板,按每组6复孔分为三组:(1)单纯肝星状细胞培养组;(2)AdGFP对照组:肝星状细胞培养24h后取病毒AdGFP以感染复数50感染肝星状细胞;(3)AdIL-10组:肝星状细胞培养24h后取病毒AdIL-10以感染复数50感染肝星状细胞。3d后分别收集各组肝星状细胞,提取总RNA。结果:与单纯肝星状细胞培养组相比,AdIL-10组的IL-10mRNA表达明显增强(P<0.01),而AdGFP对照组的IL-10mRNA表达差异无统计学意义(P>0.05);AdIL-10组的Ⅰ型胶原和Ⅲ型胶原mRNA表达明显降低(P<0.01),而AdFP对照组的Ⅰ型胶原和Ⅲ型胶原mRNA表达差异无统计学意义(P>0.05)。结论:重组腺病毒AdIL-10感染肝星状细胞后可抑制肝星状细胞Ⅰ、Ⅲ胶原分泌。更多还原

【Abstract】 Objective To investigate effects of IL-10 transgene into HSCs on the secretary of type Ⅰcollagen and type Ⅲ collagen using recombinant replication deficient adenoviruses carrying rat IL-10 cDNA(AdIL-10).Methods Primary cultured HSCs were seeded on 24-well plates at a density of 2×105 cells per well.HSCs were subjected to different treatment described as control group,AdGFP group(HSCs infected with AdGFP at a dose of MOI 50)and AdIL-10 group(HSCs infected with AdIL-10 by MOI 50).At the end of culture,total RNA was isolated from HSCs.Results IL-10 mRNA expression of AdIL-10 group was much higher than control group(P<0.01),while such expression remained the same in AdGFP group and control group(P>0.05).The changes of type Ⅰ and type Ⅲ collagen expressions of AdIL-10 group were much lower than control group(P<0.01),while such expression remained the same in AdGFP group and control group(P>0.05).Conclusion AdIL-10 could infect HSCs and decrease type Ⅰcollagen and type Ⅲ collagen mRNA expression.更多还原