【摘要】 目的:比较食管鳞状细胞癌组织、癌旁组织和正常食管组织中线粒体DNA(mitochondrial DNA,mtDNA)含量及拷贝数量的差异,探讨mtDNA与食管鳞状细胞癌发生、发展的关系。方法:应用半定量PCR法分别扩增62例食管鳞状细胞癌组织及其相应的癌旁组织和正常食管组织中的mtDNA编码区,琼脂糖凝胶电泳比较3种组织中mtDNA含量的差异;利用荧光定量PCR技术定量检测上述3组样品中的mtDNA拷贝数,并采用琼脂糖凝胶电泳对mtDNA含量进行定性检测。结果:食管鳞状细胞癌组织、癌旁组织及正常食管组织中mtDNA的检出率均为100%(62/62),但食管鳞状细胞癌组织中mtDNA相对含量高于癌旁组织及正常食管组织(P<0.05)。食管鳞状细胞癌组织、癌旁组织和正常食管组织中mtDNA的平均拷贝数分别为(26.79±0.92)×106、(26.43±0.96)×106和(25.42±0.85)×106,3组间比较差异有统计学意义(F=37.532,P<0.05)。2种PCR结果一致。结论:mtDNA拷贝数量的增加与食管鳞状细胞癌的发生、发展密切相关。更多还原

【Abstract】 Aim:To compare the number of mitochondrial DNA (mtDNA) copies among esophageal squamous cell carcinoma(ESCC),paraneoplastic mucosa and normal mucous membrane of the esophagus so as to study the relationship between mtDNA and the occurrence and development of ESCC.Methods:mtDNA copies of ESCC,paraneoplastic mucosa, and normal esophageal tissue from 62 cases of ESCC were tested using semi-quantitative PCR and real-time fluorescence quantitative PCR and PCR products were validated by agarose gel electrophoresis.Results:The detection rate of mtDNA was 100%(62/62) in the above 3 tissue. The relative contents of mtDNA in ESCC tissue were higher than those in paraneoplastic and normal esophageal tissue(P<0.05). The mtDNA copies were (26.79±0.92)×106,(26.43±0.96)×106,and(25.42±0.85)×106 in ESCC,paraneoplastic mucosa, and normal esophageal tissue, and there was significant difference(F=37.532, P<0.05). Conclusion: Increase of mtDNA copies is closely related with the occurrence and development of ESCC.更多还原