【摘要】 利用好氧反硝化细菌强化生物陶粒反应器处理含硝氮废水,探讨了生物陶粒反应器中好氧反硝化生物脱氮的实现过程,并运用变性梯度凝胶电泳技术(DGGE)对微生物菌群结构稳定性进行了分析.结果表明,在水力负荷0.75 m/h,气水比5∶1~10∶1,水温15~23℃,进水COD负荷1.92~5.98 kg/(m3.d),硝氮负荷0.60~1.34 kg/(m3.d)的条件下,生物陶粒反应器在稳定运行阶段可以基本实现对硝酸氮的完全去除,对总氮的去除率最高可达95.73%,出水中亚硝酸盐一直保持在较低水平.PCR-DGGE指纹图谱显示,微生物多样性与废水的处理效果出现协同变化的特征.在整个运行阶段,好氧反硝化菌群X31在反应器中稳定存在,并始终是优势菌群.更多还原

【Abstract】 Biological treatment of nitrate wastewater with aerobic denitrifiers in a bio-ceramic reactor was investigated.And denaturing gradient gel electrophoresis（DGGE） method was used for analyzing the stability of microbial community structure.At the condition of the influent with hydraulic loading 0.75（m/h）,gas/water ratio（5∶1）～（10∶1）,temperature 15～23℃,COD loading rate 1.92～5.98（kg/（m³·d）） and NO^-₃-N loading rate 0.60～1.34（kg/（m³·d））,high nitrate nitrogen removal of almost 100% and the maximum total nitrogen removal of up to 95.73% were achieved during stable operation stage.In addition,the nitrite concentration of effluent was lower.The PCR-DGGE profiles showed that shift of microbial diversity corresponded to the effect of nitrate removal and the preponderant populations for aerobic denitrification were steady during the test period.更多还原