【摘要】 采用高效液相色谱法进行含量测定。色谱柱为Agilent Hypersil C18(25cm×4.6mm,5μm),以0.005mol/L四丁基氢氧化铵溶液(取40%四丁基氢氧化铵6.6mL,加水1800mL,用磷酸调pH至4.0,再加水稀释至2000mL)-乙腈(720∶280)为流动相;检测波长为220nm。头孢哌酮钠、舒巴坦钠两种成分的回收率分别为100.9%、100.6%,RSD分别为0.31%、0.51%(n=6)。该方法简便,结果准确可靠。更多还原

【Abstract】 To establish HPLC for determination of contents in cefoperazone sodium and sulbactam sodium for injection. The analytical column was Agilent Hypersil C18 column (25 cm×4.6 mm,5 μm). The mobile phase was consisted of 0.005 mol/L tetrabutyl ammonium hydroxide (take 40% tetrabutyl ammonium hydroxides ,add 1 800 mL water,adjusted to pH 4.0 with phosphoric acid,eventually use water to dilute till 2 000 mL)and acetonitrile(720∶280).Detection wavelength was 220 nm. The average recovery (n=6) were 100.9% for cefoperazone sodium (RSD=0.31%) and 100.6% for sulbactam sodium (RSD=0.51%). The method was simple,accurate and reliable.更多还原