【摘要】 通过PCR—RFLP与免疫组织化学方法,研究过氧化物酶体增殖物激活受体（PPAR）γ₂基因Prol2Ala多态性与糖尿病（DM）易感性的关系,以及人皮下脂肪组织中PPARγ₂的表达与DM的相关性.实验结果表明,DM患者基因型Pro/Ala为9%,而对照组Pro/Ala为5%,DM患者与对照组之间Prol2Ala多态性频率存在差异,但不显著;用SABC方法处理皮下脂肪组织后,DM患者与正常对照组切片染色不同.PPARγ₂可能会在DM的诊断和治疗过程中起到一定作用.更多还原

【Abstract】 In this experiment,study the relationship between peroxisome proliferator-activated receptor（PPAR）γ₂ gene Pro12Ala and the susceptibility of the diabetes mellitus（DM）as well as the connection between the expre-ssion of PPARγ₂ in subcutaneous fat tissue and the DMby the PCR—PFLP and SABC method.The results demonstrated the genotype frequencies for Pro/Ala were 9% respectively in the DM group,while 5% in the control subjects.The Pro/Ala frequencies were not significantly higher in the patients than in the control group （P>0.05）;After treating with the SABC method,there were differences in dye of tissue section between patients and control subject.The difference in dye displayed that the content of expression of PPARγ₂ in subcutaneous fat tissue probably had the function of indication in diagnosis and treatment of DM.更多还原