【摘要】 目的探讨油松花粉的遗传毒性,为其应用提供安全性毒理学评价依据。方法用鼠伤寒沙门细菌营养缺陷型突变株TA97(a)、TA 98、TA 100和TA 102,采用平皿掺入法进行Ames实验,将实验分为加和不加代谢激活系统S9 2组平行实验。受实物设5个剂量组(0.008、0.040、0.200、1.000、5.000 mg/皿)。应用小鼠骨髓嗜多染红细胞微核实验,检测小鼠骨髓嗜多染红细胞微核率;利用小鼠精子畸形实验,观察不同浓度的油松花粉致小鼠精子畸形的数目。结果在Ames实验中,油松花粉各剂量组引起的回变菌落数未超过对照组自发回变菌落数的1倍以上;小鼠骨髓嗜多染红细胞微核实验显示,油松花粉3个剂量组的微核发生率均在正常范围内,与阴性对照组比较差异无显著性(P>0.05),与阳性对照组比较差异显著(P<0.05);小鼠精子畸形实验显示,油松花粉3个剂量组的精子畸形率均在正常范围内,与阴性对照组比较差异无显著性(P>0.05),与阳性对照组比较差异显著(P<0.05)。结论油松花粉对所实菌株、小鼠体细胞及生殖细胞无诱变性。更多还原

【Abstract】 Objective To investigate the genetic toxicity of pollen pini in order to assess its safety.Methods Ames Test was conducted by using histidine requiring Salmonella typhimurium indicator strains TA97(a),TA98,TA100 and TA102.The arginine esterase was detected by plate incorporation assay with and without metabolic activation system S 9 mixture at five concentrations,namely,0.008,0.040,0.200,1.000,5.000/plate.Cell micronucleus test of marrow was used.The micronucleus polychromatic erythrocytes of marrow were observed and measured.The sperm shape abnormality test was performed to assess the deform numbers of sperm.Results In Ames test,the mutagenicity of pollen pini group was less than 2 times,as compared with that of control group.There were no significant difference in micronucleus rate and deform numbers of sperm between pollen pini groups and negative control group(P>0.05),but there was a significant difference between pollen pini groups and positive control group(P<0.05).Conclusion The pollen pini is not mutagenic to tested trains,somatic cell and male germ cells in mice.更多还原