【摘要】 目的研究口服伤寒杆菌后,大鼠小肠和大肠上皮内淋巴细胞(IEL)的形态学特征及分布特点的变化,为探讨粘膜免疫的机制打下基础。方法给大鼠口服伤寒杆菌,应用免疫组化染色和电镜的方法对大鼠消化道各部分的黏膜 IEL 表型进行分析,观察其形态特征。结果电镜观察显示大鼠被免疫后肠道淋巴细胞超微结构与对照组相比功能活跃;免疫组化观察到大鼠被免疫后肠道各段IEL 的亚群数量明显增加,消化管各部 IEL 数量有差异。结论服伤寒杆菌后,大鼠小肠和大肠 IEL活性明显增强,是免疫反应的形态基础。IEL 数量的大量增加,进一步表明淋巴细胞在粘膜免疫中起重要作用。更多还原

【Abstract】 Objective To study the differences of distributed and morphological features between intraepi- thelial lymphocyte(IEL)of small intestine(duodenum,jejunum,ileum)and large intestine(sigmoid colon) in rat that after taking Salmonella typhi from mouth.The purpose was to play foundation of researching the rule of mucosal immunity.Methods First rats were taken Salmonella typhi from mouth,sthen analyzed the surface mark of IEL morphological feathers of them by immunohistochemistry staining and electron micro- scope.Results Observation from microscope:The ultra structure of lymphocyte display that the fuction of experimental group more active compare with control group after immunjty.The amount of various subsets of IEL invarious parts of rat’s intestine increased obviously by immunohistochemistry staining.Conclusions The activation becomed obvious after taking Salmonella type,it was the foundation of immune response.更多还原