【摘要】 将半抗原醋酸甲孕酮(MPA)衍生物与牛血清白蛋白(BSA)偶联后,作为包被原与游离的醋酸甲孕酮竞争抗MPA多克隆抗体,通过该模式建立间接竞争ELISA方法检测MPA的残留量。试验结果表明:理想的包被抗原为0.625μg/ml,抗体的工作浓度为1∶8000,标准曲线在1~50 ng/ml之间线性关系较好,最低检测限0.1 ng/ml,得回归方程y=-1.1479x+0.9426(r2=0.9679),批内和批间变异系数分别为5.35%、10.89%。更多还原

【Abstract】 Coupled with the BSA,hapten MPA,used as a coating antigen,combined with anti-MPA polyclonal antibody with the competition of free MPA.Indirect competition ELISA was set up according to above-mentioned mode to detect the residue level of MPA in the example.The results of the experiment demonstrates that the optimal concentration of the coating antigen was 0.75 μg/ml.Polyclonal antibody against MPA was 1∶8000 and the standard curve had a good linearity in the rang of 1~50 ng/ml with detection limit being 0.1 ng/ml.The regression equation y=-1.1479x+0.9426(r2=0.9679) were obtained while the coefficient of variation of intra-assay and extra-assay were 5.35%,10.89% respectively.更多还原