【摘要】 文章根据ZrOCl2水解产生的[ZrO(OH)]+能与果糖作用产生荧光的原理,建立了果糖的定量测定方法。在NH4Cl介质中,体系的荧光强度有所增强,产物在408nm处发荧光,在0.5～25μg·mL-1范围内荧光强度与果糖浓度成正比,当反应温度为70℃、反应时间15min时,葡萄糖不干扰测定。该法成功用于水果中果糖的测定,灵敏度高,检出限达0.071μg·mL-1,方法稳定性好,结果满意。更多还原

【Abstract】 According to the principle that Zn(OH)+ produced by ZrOCl2 upon hydrolysis in water can react to form a fluorescent derivative with fructose, a method for the quantitative determination of fructose has been proposed. The fluorescence of the derivative enhanced at 408 nm in NH4Cl medium has a direct ratio with fructose in the linear range of 0.5-25 μg·mL-1. When reaction conditions such as a temperature of 70 ℃ and a time of 15 min are used, the detection limits is 0.071 mg·mL-1. The proposed method was also applied successfully to the determination of fruit, satisfaction with high accuracy and good reproduction.更多还原