【摘要】 测量了分属硅藻和甲藻两个门类的六种我国东海常见赤潮藻的叶绿素荧光激发光谱,以Matlab为操作平台,采用自编的光谱求导程序,对荧光激发光谱进行四阶导数处理。结果表明,四阶导数法能有效去除光谱中的细小杂峰,并将重叠的色素荧光峰分离,实现主要色素的定性定量分析。四阶导数光谱在350～550nm波长范围内出现的6个极大值主要代表了活体藻液中的非色素荧光物质、叶绿素和类胡萝卜素的荧光峰。通过原始叶绿素荧光激发光谱和四阶导数光谱的对比分析,表明四阶导数光谱法能够凸显硅藻和甲藻之间的光谱差异,增强光谱识别硅藻和甲藻的能力,同时也提高了光谱的稳定性和重现性。更多还原

【Abstract】 Chlorophyll fluorescence excitation spectra of six phytoplankton species, belonging to Bacillariophyta and Dinophyta, were dealt by fourth-derivative analysis with the Matlab program. The results show that between 350 nm and 550 nm six fluorescence peaks were found in the fourth-derivative spectra, which are representatives of non-pigments, chlorophylls and carotenoides respectively. The method makes Bacillariophyta and Dinophyta more distinguishable when the fourth-derivative spectra are compared with the chlorophyll fluorescence excitation spectra. It can be used not only to discriminate the two groups of algaes, but also to reduce the effect of noise. The fluorescence peaks in the fourth-derivative spectra are proved to be stable.更多还原