【摘要】 研究羟基磷灰石纳米颗粒(HAp nanoparti-cles)引起巨噬细胞凋亡,及其与热休克蛋白Hsp70的相关性。THP-1单核细胞株经PMA诱导成为巨噬细胞,分别加入0、20、100μg/ml HAp纳米颗粒,培养24h后经PI和AnexinV染色,在荧光显微镜下观察,用流式细胞仪检测细胞的凋亡情况,应用western blot方法检测Hsp70蛋白分子表达的改变。显微镜观察显示THP-1单核细胞株能被诱导成巨噬细胞。荧光显微镜显示20、100μg/ml HAp纳米颗粒组凋亡的阳性信号明显强于0μg/ml对照组;流式细胞仪检测结果也证实HAp纳米颗粒组比对照组凋亡率明显增高(P1<0.0001,P2<0.0001),且凋亡的程度呈浓度依赖性(P3=0.025)。Western Blot检测显示20、100μg/ml HAp纳米颗粒组较对照组Hsp70蛋白表达增加,并且随颗粒浓度增高而表达增加。更多还原

【Abstract】 To explore whether HAp nanoparticles induce apoptosis of macrophages and its correlation to Hsp70.Induce THP-1 cell lines to become macrophage with PMA.Co-culture the macrophages with 0,20,100μg/ml HAp nanoparticles in medium for 24h.Collect the macrophages,and dye with PI and AnexinV, then observe and detect the rate of apoptosis of the macrophages;determine the expression level of Hsp70 by Western Blot.THP-1 cell lines can be induced to macrophages.Detection for apoptosis shows that: observing through fluorescence microscope,positive apoptosis signal of 20,100μg/ml HAp particles groups are stronger than that of control group;detecting by flow cytometry(FCM),apoptosis rate of 20,100μg/ml HAp particles groups are also higher than that of control group(P1<0.0001,P2<0.0001),moreover,the rate depends on the concentration of HAp nanoparticles(P3=0.025).Detection by Western Blot result shows the expression of Hsp70 of 20,100μg/ml HAp particles groups increase comparing to control group,and the level also depends on concentrations of HAp nanoparticles.更多还原