【摘要】 目的:分析中国人诱导型一氧化氮合成酶G-954C等位基因位点的基因表型在糖尿病视网膜病变黄斑囊样水肿患者与正常对照的差异。方法:病例对照,89例糖尿病视网膜病变黄斑囊样水肿的患者与年龄及性别匹配的90例健康对照纳入该项研究。研究采用了巢式聚合酶链式反应、限制性核酸内切酶技术、基因片段的序列测定。主要检测指标为诱导型一氧化氮合成酶G-954C位点的基因表型。结果:89例患者和90例健康对照的诱导型一氧化氮合成酶G-954C位点的基因表型全部检测结果为GG型。结论:诱导型一氧化氮合成酶G-954C位点的基因多态性可能在中国人为较低的变异频率,而且可能与糖尿病视网膜病变黄斑囊样水肿的易感性关系不大。更多还原

【Abstract】 AIM:To analyze the genotype of the allele distribution of a polymorphic G-954C within the 5’ upstream promoter region of the nitric oxide synthetase 2A gene(NOS2A) in samples of diabetic retinopathy in patients with cystoid macular edema in the mainland of China.METHODS:Eighty-nine patients with diabetic retinopathy and cystoid macular edema and 90 healthy controls were enrolled in this study.Nest polymerase chain reaction(PCR) was performed,and restriction endonuclease digestion and gene fragments sequence were examined to detect the genotype of NOS2A G-954C.RESULTS:The genotypes of the sample population of 89 cases and 90 healthy controls were all detected as GG.CONCLUSION:The distribution of G-954C of NOS2A polymorphism are at a lower frequency in China,with little relevancy to the frequency of diabetic retinopathy combined with cystoid macular edema.更多还原