【摘要】 目的观察内皮素抗体对大鼠脑出血致应激性溃疡实验动物模型干预的作用和效果。方法将SD大鼠分为对照组(A组)、模型组(B组)、内皮素抗体组(C组)各10只。用非精确定位穿刺法将实验组大鼠自体尾静脉血200μl注入右脑实质内,分别于尾静脉注射生理盐水、内皮素抗体,1次/d,72h处死。观察胃黏膜溃疡发生率,溃疡指数,血清内皮素,血清、脑、胃黏膜的MDA、SOD,脑、胃黏膜光镜和电镜的病理变化及超微结构电脑图像定量分析。结果B组胃黏膜溃疡发生率为30%,溃疡指数为15,其他各组均未见肉眼溃疡灶。与B组比较,C组血清、脑、胃黏膜的MDA、SOD以及脑含水量均有统计学意义(P<0.01);C组脑神经元细胞、胃壁细胞超微结构电脑图像定量分析中的线粒体比表面(Rsv)、体积密度(Vv)差异无统计学意义(P>0.05);3组间血清内皮素差异无统计学意义(P>0.05)。结论内皮素抗体干预可有效减轻大鼠脑出血致应激性溃疡的发生和发展。更多还原

【Abstract】 Objective To study the effects of endothelin antibody on acute stress ulcer sencondary to cerebral hemorrhage in rats.Methods Thirty SD rats were randomly divided into control group(group A,n=10),experimental group(group B,n=10) and endothelin antibody(group C,n=10).Rat right intracerebral hemorrhage model was established by injection of 200 μl autologous caudal venous blood.Normal saline or endothelin antibody were administered intravenously dialy,and rats were sacrificed at 72 hours of the experiment.The incidence of acute stress ulcer and ulcer index were observed and serum endothelin level,MDA content and SOD activity in serum,brain and gastric mucosa were measured,Brain tissue and gastric mucosa were examined by light microscopy and electron microscopy.Results Acute stress ulcer was only observed in group B(the incidence was 30%,ulcer index 15).Tissue MDA content and SOD activity in groups C were significantly different from group-B(P<0.01),Cerebral water content in groups C were significantly different from group B(P<0.01),Rsv and Vv in neuron and gastric parietal cell of group C were not statistically different with group B(P>0.05).Serum endothelin level were not statistically difference among the three groups(P>0.05).Conclusion Endothelin antibody can reduce occurrence and development of acute stress ulcer sencondary to cerebral hemorrhage in rats.更多还原