【摘要】 采用HPLC-UV与反相C18柱,以50 mmol/L的KH2PO4(pH 5.5)-10%的甲醇(V/V)作为洗脱液,同步快速检测了21位健康人与90位病人胃粘膜活检组织中的脱氧鸟苷(deoxyguanosine,dG)和8-羟基脱氧鸟苷(8-Hydroxydeox-yguanosine,8-OHdG)的量。方法线性范围分别为3.0~400μg/mL、0.2~10μg/mL,检出限可分别达到1.0、0.1μg/mL(S/N=3)。实验发现病人胃粘膜活检组织中的8-OHdG量远远高于健康人,此结果在临床早期诊断相关疾病方面具有很高的应用价值。更多还原

【Abstract】 8-Hydroxydeoxyguanosine(8-OHdG) has been widely used as a biomarker of oxidative DNA damage in livings.However,the levels of 8-OHdG aren′t available on other exposure such as human gastric mucosal biopsy.In this paper,we simultaneously investigated the levels of 8-OHdG and dG in DNA from human gastric mucosal biopsy with HPLC-UV.The analysis was performed on a RP-18 analytical column using potassium phosphate buffer(50 mmol/L,pH 5.5)-methanol(90/10,V/V) as eluent.The calibration graph for 8-OHdG was linear in the concentration range of 0.2～10 μg/mL,and the limit of detection was 0.1 μg/mL(signal to noise ratio S/N=3).The concentration of 8-OHdG in 21 healthy persons and 90 patients′ gastric mucosal biopsy was determined.It was found that levels of 8-OHdG in patients was significantly higher than those in healthy persons(p<0.001),the results demonstrated that 8-OHdG may be a good marker for diagnosis of some illness.更多还原