【摘要】 以表没食子儿茶素没食子酸酯(Epigallocatechin-gallate,EGCG)为模板分子,α-甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,在光冷引发条件下合成EGCG分子印迹聚合物,利用该聚合物制成分子印迹固相萃取柱,用于固相萃取茶叶提取物茶多酚,对萃取柱中的清洗液、洗脱剂、上载量等进行了选择。结果表明,在萃取柱上载样品之后,先用V(甲醇)∶V(水)=1∶9溶液进行清洗,再用V(甲醇)∶V(乙酸)=9∶1混合液进行目标分子的洗脱,可以得到比较纯的目标物质EGCG(色谱峰相对峰面积达92.4%),萃取柱在上载样品并进行清洗、洗脱之后,EGCG的回收率达69.3%。分子印迹柱具有较好的稳定性和耐用性能,使用20次后其选择性识别能力仍未降低,但空白印迹柱却没有这样的选择性识别能力。更多还原

【Abstract】 A molecularly imprinted polymer(MIP) was prepared under solicitation condition with ray and freeze using methacrylic acid as monomer,Epigallocatechin gallete (EGCG) as template molecule and ethylene glycol dimethacraylate as the cross-linking agent.Solid-phase extraction column was prepared with MIP as solid-phase material and used for extraction of tea polyphenols from green tae,cleaning agent and eluent and uploading quantity were selected.The optimum conditions to the column were as following: the sample was uploaded,and then the column was cleaned up with methanol-water(1∶9,V/V) solution.EGCG was eluted with methanol-acetic acid(9∶1,V/V) and pure EGCG was obtained(percentage of relative area of chromatographic peak reached 92.4%).The result showed that the EGCG recovery was 69.3%.solid-phase extraction column with MIP as solid-phase material had good stability and durability and its recognition ability didn′t descend when the EGCG-MIP column was used 20 times.However,blank solid-phase extraction column without MIP was not as good as MIP column.更多还原