【摘要】 用荧光光谱法研究了人体生理pH条件下,3-甲基-6-氨基-5-氰基-4-(2-邻羟基苯基)-1-苯基-1,4-二氢吡喃并[2,3-c]吡唑(I)与牛血清白蛋白(BSA)间的结合反应。获得了不同温度下I与BSA作用的结合常数K和结合位点数n,计算给体-受体间距离r为5.75 nm及能量转移效率E为0.74。证实了I与BSA的相互结合作用为单一的静态猝灭过程,结合力以疏水作用力为主。更多还原

【Abstract】 The interaction of 6-amino-1,4-dihydro-4-(2-hydroxyphenyl)-3-methyl-1-phenylpyrano pyrazole-5-carbonitrile(I) with bovine serum albumin(BSA) was studied under physiological pH conditions by fluorescence spectrometry.The binding constants(K) and binding sites(n) at different temperatures were obtained,and the distance r(5.75 nm) and energy transfer efficiency E(0.74) between donor and acceptor were also determined.It indicated that the binding reaction of I with BSA was a single static quenching process and the existed strong binding interaction was mainly hydrophobic interaction.更多还原