【摘要】 目的观察骨关节炎（OA）成纤维样滑膜细胞（FLS）体外培养时生长增殖特性,以及白芍总苷（TGP）对其增殖能力的影响,探讨白芍总苷的抗炎机制。方法四甲基偶氮唑蓝（MTF）法和反转录-聚合酶链反应（RT-PCR）方法观察OA和正常对照（NS）膝关节滑膜组织体外培养时FLS增殖能力和c-fos表达情况,酶联免疫吸附试验（ELISA）法检测培养上清中的肿瘤坏死因子（TNF）-α、干扰素（IFN）-γ和碱性成纤维细胞生长因子（bFGF）,用流式细胞仪测定FLS细胞周期的变化。结果OA和NS的FLS在体外培养时的细胞倍增时间差异无统计学意义。加入高剂量TGP对OA-FLS细胞存活分数（SF）的抑制作用较NS-FLS组更显著（P<0.05）,2000 mg/L和400 mg/L TGP降低培养液上清中TNF-α和bFGF的水平,升高IFN-γ含量（P<0.05）,并抑制FLS的c-los mRNA表达（P<0.05）。OA-FLS细胞周期测定表明加入2000 mg/L的TGP可延长G₁期而缩短S期,但与不加药对照组相比差异无统计学意义（P>0.05）。结论高浓度TGP对OA-FLS的增殖能力、细胞因子分泌及原癌基因表达的抑制程度大于对正常滑膜FLS的作用,表明TGP调节FLS增殖特性,有抑制OA滑膜炎的作用。更多还原

【Abstract】 Objective To investigate the proliferative characteristics of fibroblast-like synovial cells （FLS）in osteoarthritis in vitro and the mechanism of the immunosuppressive effect of total glucusides of paeony（TGP）.Methods FLS of OA and non-inflamed synovium（NS）were cultured and identified in vitro in the presence or absence of TGP.After incubation,the survival fraction（SF）of FLS was evaluated by MTI’ and the TNF-α,IFN-γand bFGF level in cultured FLS supernatant was measured by ELISA.The expression of FLS c-los mRNA and cell cycle of OA-FLS was observed by RT-PCR and flow eytometry respectively at the same time.Results No statistical significant differences were noted between the OA and NS FLS in pro- liferating double time.High doses of TGP suppressed FLS-SF more evidently in OA patients than in NS（P<0.05）.The SF of FLS interfered by TGP was lower than that of no drug control（P<0.05）.The TNF-α,bFGF level in OA-FLS supernatant was depressed and IFN-γwas increased,the accumulation of c-fos mRNA was reduced when FLS cultured with 2000 mg/L or 400 mg/ml TGP（P<0.05）.The TGP could change the cell cy- cle of FLS,prolong G₁ phase and shorten S phase at dose of 2000 mg/ml,but no statistical significant differ- ences were observed（P>0.05）.Conclusion High dose TGP can inhibit OA-FLS proliferation,modulate cy- tokine secretion and c-fos expression in OA.This suggests that TGP has immunosuppressive effect on OA syn- ovitis,probably by preventing the synovial hypertrophy in OA.更多还原