【摘要】 目的:应用蛋白质组学方法筛选子痫前期差异蛋白,探讨差异蛋白与子痫前期发生的可能关系.方法:收集5例正常足月妊娠妇女和5例足月子痫前期重度患者胎盘母面及宫壁上的蜕膜组织,提取总蛋白.双向凝胶电泳技术对总蛋白进行分离,考马斯亮兰染色,获得蛋白质图谱.用PDQuest软件进行图像分析,寻找差异蛋白,随机选取8个差异蛋白质点进行胶内酶解,用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)技术获得肽质量指纹谱,搜寻蛋白质数据库鉴定蛋白质并进行初步分析.结果:获得了正常妊娠及子痫前期重度患者蜕膜组织双向电泳凝胶图谱,蛋白质点子痫前期组为(436±13)个点,正常妊娠组为(425±16)个点,图像分析结果显示表达丰度相差2倍以上的差异蛋白质点有14个,它们在子痫前期中均表现下调.随机选取的8个蛋白质点均成功得到鉴定,它们分别是膜联蛋白-Ⅴ,血红蛋白β链,Rho-GDP解离抑制剂1,氯离子细胞内通道蛋白1,原肌球蛋白4,平滑肌蛋白22α2,α1-抗胰蛋白酶和纤维蛋白原β链.结论:子痫前期重度患者蜕膜组织中存在差异蛋白,这些蛋白质在子痫前期的发病过程可能起重要作用.更多还原

【Abstract】 AIM:To screen the differential proteins in deciduas of pre-eclampsia and to study their roles in pre-eclampsia.METHODS:The deciduas from 5 normal women of full-term pregnancy and 5 pre-eclampsia patients were collected to obtain the total proteins.The proteins from the 2 groups underwent two-dimensional polyacrylamide gel electrophoresis(2-D PAGE) and Coomassie brilliant blue stained gel imaging with PDQuest image analysis software.The peptide mass fingerprinting(PMF) was acquired by matrix assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).The resulted data were then searched against protein databases for the identification of the proteins,and an initial analysis was made accordingly.RESULTS:2-DE patterns of the deciduas of normal full-term pregnancy group and preeclampsia group were acquired.According to them,(436±13) protein spots were seen in preeclampsia group and(425±16) in normal pregnancy group.Fourteen differential spots were down-regulated in preeclampsia group among them and 8 differential spots were all successfully identified.They were Annexin Ⅴ,Hemoglobin β chain,Rho-GDP-dissociation inhibitor 1,chloride intracellular channel protein 1,Tropomyosin α4 chain,Transgelin 22α2,α1-antitrypsin and Fibrinogen β chain,respectively.CONCLUSION:Differential proteins found in deciduas from patients with severe preeclampsia might play an important role in the pathogenic process of preeclampsia.更多还原