【摘要】 目的:探讨14-3-3σ和P53蛋白表达在食管鳞癌和癌旁正常黏膜上皮、各级癌前病变中的变化特征及相关性.方法:采用免疫组织化学方法检测14-3-3σ和P53蛋白在60例不同分化程度食管鳞癌和癌旁正常食管黏膜上皮、各级癌前病变中的表达变化.结果:癌旁正常食管黏膜上皮中均可检测到不同程度的14-3-3σ蛋白表达(表达量8.22),随着癌前病变进展,14-3-3σ的表达率和表达量逐渐下降;低分化鳞癌中14-3-3σ的表达量最低(表达量0.45),完全失表达率为64%(7/11).P53蛋白在鳞癌中表达率最高67%(27/40),表达量为4.86,而只有25%(6/24)癌旁正常上皮表达P53蛋白(表达量0.42).14-3-3σ和P53蛋白的相关性分析表明二者呈显著负相关(P<0.05).结论:P53蛋白可能通过负调控14-3-3σ基因的表达参与食管癌变多阶段演进过程,14-3-3σ和P53可能成为食管癌早期诊断及高危人群筛查的分子指标,同时也可能成为食管癌生物预防和疗效评价的新靶点.更多还原

【Abstract】 AIM: To characterize the 14-3-3σ and P53 protein expressions during multi-stage carcinogenesis of esophageal squamous cell carcinoma (ESCC) and in normal esophageal mucous epithelium. METHODS: Immunohistochemistry (ABC) methods were used to determine 14-3-3σ and P53 protein expressions in 60 cases of ESCC, nearby matched normal esophageal epithelium and a variety of ESCC precursors. RESULTS: High expression of 14-3-3σ was found ubiquitously in normal esophageal epithelium with a digitalized average value of 8.22 in expression. Protein 14-3-3σ was down-regulated stepwise during the development of esophageal carcinoma from normal epithelium. Sixty-four percent of poorly-differentiated squamous cancer lost 14-3-3σ expression with the digitalized value of 0.45. However, pattern of P53 protein expression was in contrast to 14-3-3σ. Sixty-seven percent of esophageal cancer expressed P53 at a high level with a quantitated amount of 4.8. In nearby normal epithelium of esophagus, the P53 protein expression was low with a digitalized value of 0.42. Significant negative correlation between 14-3-3σ and p53 expressions was revealed by Spearman rank correlation analysis (P<0.05). CONCLUSION: The aberrant expression pattern of 14-3-3σ and p53 may contribute to the formation and progression of ESCC and may predict the prognosis of ESCC patients. Proteins 14-3-3σ and P53 have the potential to become biomarkers for early diagnosis of esophageal cancer and end-point molecules of ESCC prevention.更多还原