【摘要】 目的了解深圳地区人乳头状瘤病毒(human papillomavirus,HPV)亚型的分布情况,并评价不同亚型的HPV在宫颈病变中的临床意义。方法用导流反斑点杂交法对深圳市人民医院2003-2004年352例尖锐湿疣标本进行9～20种HPV亚型基因分型,结合临床病理检查结果分析HPV亚型分布情况及与不同宫颈病变的关系。结果深圳地区尖锐湿疣患者中HPV亚型分布较广泛,主要亚型以低危型11和6型为主,多重HPV感染率接近总感染率的37%;在宫颈尖锐湿疣中主要的HPV亚型是高危型,尤其在子宫颈上皮内瘤样病变(cervical intraepithelial neoplasia,CIN)Ⅱ+以上的宫颈病变中,感染率最高的依次为以16、18、58、52和33型。结论深圳地区尖锐湿疣以HPV11和6感染为主。HPV16、18等高危型HPV亚型可能是深圳地区导致宫颈恶性病变主要原因。更多还原

【Abstract】 Objective To investigate the distribution of human papillomavirus(HPV) subtypes in condyloma acuminatum(CA) patients in Shenzhen People’s Hospital and assess the association between HPV subtypes and cervix neoplasm.Methods Type specific prevalence and extent of multiple infection were assessed in the genital tract CA samples collected from 352 patients visiting Departments of dermatology or gynecology of Shenzhen People’s Hospital during 2003-2004.PCR using MY09/11 as primer and reverse dot blot hybridization for the genotyping of 9-20 HPV subtypes.Results HPV type diversity was broad in the investigated CA patients.The low risk HPV type(HPV subtype 11,6) was dominant and multiple HPV infections occurred in 37% of HPV-positive samples.High risk HPV type was dominant in CA from cervix,especially in the high grade cervical intraepithelial neoplasia(CIN Ⅱ+),and multiple high risk HPV(HR-HPV) infection was found in 87% of HPV positive samples.Conclusion HPV subtypes 6 and 11 were dominant in the patients visiting Shenzhen People’s Hospital.HPV subtypes 16 and 18 may be the main causes of malignant changes of cervix.更多还原