Damaging Effect of Low Energy N~+ Implantation on Aspergillus niger Spores

【摘要】 <正> The mutant effects of a keV range nitrogen ion(N~+)beam on enzyme-producingprobiotics were studied,particularly with regard to the induction in the genome.The electronspin resonance(ESR)results showed that the signal of ESR spectrum existed in both implantedand non-implanted spores,and the yields of free radicals increased in a dose-dependent manner.The ionic etching and dilapidation of cell wall could be observed distinctly through the scanningelectron microscope(SEM).The mutagenic effect on genome indicated that N+ implantation couldmake base mutation.This study provided an insight into the roles low-energy ions might play ininducing mutagenesis of micro-organisms.更多还原

【Abstract】 The mutant effects of a keV range nitrogen ion(N~+)beam on enzyme-producing probiotics were studied,particularly with regard to the induction in the genome.The electron spin resonance(ESR)results showed that the signal of ESR spectrum existed in both implanted and non-implanted spores,and the yields of free radicals increased in a dose-dependent manner. The ionic etching and dilapidation of cell wall could be observed distinctly through the scanning electron microscope(SEM).The mutagenic effect on genome indicated that N+ implantation could make base mutation.This study provided an insight into the roles low-energy ions might play in inducing mutagenesis of micro-organisms.更多还原