【摘要】 在田间条件下,以12个不同来源大豆品种为试验材料,采用二因素裂区设计,研究了不同大豆品种间叶片氮代谢相关指标的差异,以及氮素对叶片氮代谢相关指标的影响。结果表明:氮肥处理对不同来源大豆品种叶片氮代谢指标影响不同,不同生育时期大豆对氮肥的反应也有所差别。施肥处理会降低开花期辽宁当代品种叶片的谷氨酰胺合成酶（GS）活性;中等肥力处理(100 kg hm^-2)会提高结芙期俄亥俄当代品种叶片的GS活性,而高肥处理则会降低该酶的活性;辽宁当代品种和辽宁老品种叶片GS活性随着施氮量的提高而降低。在鼓粒期,中等肥力处理会提高俄玄俄当代品种和辽宁当代品种叶片的GS活性,而施肥处理会降低辽宁老品种的GS活性。在开花期,三组不同来源品种叶片的硝态氮含量均随着施氮水平的提高而增加。施肥处理能显著提高叶片的氨态氮含量。更多还原

【Abstract】 Nitrogen is the important element for growth and development of soybean.Twelve soy- bean cultivars from different regions were used to study the effect of nitrogen fertilizer on nitro- gen metabolism eorrelation index in leaves,i.e.activity of glutamine synthetase(GS),content of nitrate-N and ammonia-N.The experiment was carried out by the split plot design in the field con- dition.The results showed that the effects of nitrogen fertilizer treatments on nitrogen metabo- lism related index in leaves of soybean cultivars from different regions were different.And the re- sponses to nitrogen fertilizer treatments varied with growth stages.The activity of GS for Liaon- ing new cultivar(LNC)was decreased by nitrogen fertilizer treatments at flowering stage.The ac- tivity of GS for Ohio new eultivar(ONC)was increased by middle level of fertilizer treatment while decreased under high level of fertilizer treatment at podding stage.The activity of GS was decreased with the fertilizer levels improved for LNC and Liaoning old cultivar(LOC)at podding stage.The activity of GS was increased by the middle level of fertilizer treatment for ONC and LNC at grain filling stage.The activity of GS was decreased by the fertilizer treatment for LOC at grain filling stage.The content of nitrate-N was increased with nitrogen level improved for three group soybean cultivars at blooming stage.The content of ammonia-N in leaf was increased by ni- trogen fertilizer treatments.更多还原