【摘要】 目的:探讨维生素B12片剂中维生素B12的含量测定方法。方法:紫外可见分光光度法。样品以标准曲线法为测定含量依据,在361nm的波长处测定吸光度。结果:维生素B12在5μg/mL～100μg/mL浓度范围线性关系良好。回归方程Y=0.0193X+0.048,r=0.9937。结论:测定的5个不同批号样品其含量均在标示范围之内,该方法简便、准确、灵敏度高。更多还原

【Abstract】 Objective:The measurement method of V-B12 content in tablet is established.Methods:UV-Vis The absorption is measured at 361 nm according to calibration carve method.Results:The linear range is in 5 μg/mL～100 μg/mL and the regression equation is Y=0.0193X+0.048,r=0.9937.Conclusion:The measurement is done in 5 kind of lot number and the result indicate that the V-B12 content in tablet corresponds with standard range. This method is simple, accurate and high sensitivity.更多还原