【摘要】 采用3只装有永久性瘤胃瘘管的萨能母山羊为试验动物,试验分为4期,每期6d,每期为一个处理。对照组:基础日粮;处理Ⅰ:基础日粮+15g蛋氨酸;处理Ⅱ:基础日粮+15g蛋氨酸+0.15g拉沙里菌素;处理Ⅲ:基础日粮+15g蛋氨酸+0.20g拉沙里菌素。每期的最后一天为血样的采样日,采样日的采样时间点为3个,即当日早晨饲喂前(0h)、饲喂后2h和5h。血样经处理后,测定样本中血浆游离蛋氨酸(PFM)的含量和有关酶的活性。试验结果表明:不同处理之间,在0h处理Ⅲ的谷氨酸脱氢酶(GLDH)、γ-谷氨酰转移酶(GGT)活性显著高于对照组(P<0.05),谷草转氨酶(GOT)极显著低于对照组(P<0.01),谷丙转氨酶(GPT)活性极显著高于对照组(P<0.01),处理ⅡGOT活性极显著低于对照组和处理Ⅰ(P<0.01);在2h处理ⅡGGT活性显著低于处理Ⅲ(P<0.05),极显著低于处理Ⅰ(P<0.01),处理ⅢGPT活性显著高于处理Ⅱ(P<0.05),极显著高于对照组和处理Ⅰ(P<0.01);在5h处理ⅠGGT活性显著高于处理Ⅱ(P<0.05),处理ⅢGPT活性极显著高于对照组(P<0.01),处理Ⅰ的PFM水平在0、2、5h均极显著高于对照组、处理Ⅱ和处理Ⅲ(P<0.01);不同处理内,处理Ⅰ的0hGGT活性显著低于2、5h(P<0.05),5h的PFM水平显著高于0、2h(P<0.05),处理Ⅲ的5hGGT活性低于0h(P<0.05)。更多还原

【Abstract】 3 goats fitted with fistula in the rumen.Experiment included 4 periods.Every period which was a treatment was 6 days.The treatments in the experiment were based diet plus 0(Control group,CG),15g Met(Treatment Ⅰ,TⅠ),15g Met and 0.15g lasalocid(Treatment Ⅱ,TⅡ),15g Met and 0.20g lasalocid(Treatment Ⅲ,TⅢ).Sampling time was at 0 hour(morning before feeding),2 hours(morning after feeding),and 5 hours(morning after feeding)in the last day of every period.The content of plasma free methionine(PFM)and related enzymatic activties of samples were determined.The results showed that glutamic dehydrogenase(GLDH)and γ-Glutamyltransferase(GGT)activites of TⅡ in 0 hour were higher than that of CG(P<0.05),and glutamic oxaloacetic transaminase(GOT)activity of CG was significantly higher than that of TⅡ(P<0.01)in 0 hour,and glutamic pyruvate transaminase(GPT)activity of TⅡ in 0 hour was significantly higher than that of CG(P<0.01),and GOT activity of CG and TⅠ in 0 hour was significantly higher than that of TⅡ(P<0.01),and GGT activity of TⅢ in 2 hours was higher than that of TⅡ(P<0.05),and GGT activity of TⅠ in 2 hours was significantly higher than that of TⅡ(P<0.01),and GPT activity of TⅢ in 2 hours was significantly higher than that ofTⅡ(P<0.05),CG and TⅠ(P<0.01),and GGT activity of TⅠ in 5 hours was higher than that of TⅡ(P<0.05),and GPT activity of TⅢ in 5 hours was significantly than higher that of CG(P<0.01),and the content of PFM of TⅠ in 0,2 and 5 hours was significantly higher than that of CG,TⅡ and TⅢ(P<0.01);and GGT active of CG in 2 and 5 hours was significantly higher than that of CG in 0 hour,and the content of PFM of CG in 5 hours was higher than that of CG in 0 and 2 hours,and GGT activity of TⅢ in 0 hour was higher than that of TⅢ in 5 hours.更多还原