【摘要】 目的探讨髓样分化蛋白(myeloid differentiation protein-2,MD2)在内毒素(LPS)激活肺泡Ⅱ型上皮细胞(ATⅡcells)中的表达及可能作用机制。方法通过RT-PCR的方法检测MD2mRNA在ATⅡcells的表达;电泳迁移率改变法检测LPS刺激后的ATⅡcellsNF-κB活性的变化;ELISA检测ATⅡcells培养上清中TNF-α和IL-6的含量变化。结果正常ATⅡcells表达有MD2mRNA,LPS刺激后表达增强;其表达在1～103ng/mlLPS范围内呈浓度依赖性增强。LPS刺激后,ATⅡcellsNF-κB活性及TNF-α和IL-6的生成均呈浓度依赖性的增强。结论MD2在LPS激活ATⅡcells中起着重要作用。更多还原

【Abstract】 Objective To investigate the expression and roles of myeloid differentiation protein-2(MD2) in the activation of alveolar typeⅡepithelial cells(ATⅡcells) induced by lipopolysaccharide(LPS).Methods The expressions of MD2 mRNA in ATⅡcells were detected by semi-quantitative RT-PCR.The activity of NF-κB in ATⅡcells was measured with electrophoretic mobility shift assay(EMSA).The TNF-α and IL-6 concentrations in the supernatant of cultured ATⅡcells were tested with ELISA.Results The expressions of MD2 mRNA were detected in normal ATⅡcells.After stimulation with LPS,the expressions increased in a dose-dependent manner in the range of 1-103ng/ml LPS.In ATⅡcells after stimulation with LPS,the NF-κΒ activity and the production of TNF-α and IL-6 increased in a dose-dependent manner.Conclusion MD2 play a pivotal role in the activation of ATⅡcells induced by LPS.更多还原