【摘要】 目前检测重金属使用的国标方法灵敏度高、特异性强,但存在仪器费用高等缺陷。为了建立快速、简便、只要比色即可测定镉、锡、铅的酶抑制法,选择葡萄糖氧化酶(GOD)—辣根过氧化物酶(POD)催化体系,优化了试验条件;利用金属离子对酶活的抑制作用,测定了重金属离子镉、锡、铅对葡萄糖氧化酶活力的影响。所测镉、锡、铅检出限分别为1.3,0.4,1.4μg/mL。研究了检测实际样品中镉、锡、铅的方法,加标回收率分别为92.7%~105.7%;标准差≤1.0%。更多还原

【Abstract】 The presently used GB method for detecting heavy metals is high sensitivity and selectivity,however,the equipments are expensive.This paper aims at establishing a quick,convenient enzyme inhibition method which needs only vis-spectphotometry to detect Cd,Sn,and Pb.The effects of metal ions on the activity of glucose oxidase(GOD)-peroxidase(POD) was deteded by using the restraining effect.The detection limits of Cd,Sn,and Pb are 1.3 μg/mL,0.4 μg/mL,and 1.4 μg/mL,respectively.The rate of recovery of the metal ions ranged from 92.7% to 105.7%,and RSD%≤1.0% in the samples.更多还原