【摘要】 为探讨青年猫和老年猫小脑皮质GABA能神经元及其表达的年龄相关性变化,利用Nissl染色显示小脑皮质结构及神经元,免疫组织化学ABC法标记GABA免疫阳性神经元。光镜下观察,采集图像,并利用图像分析软件对分子层、蒲肯野细胞层和颗粒层神经元及GABA免疫阳性神经元及其灰度值进行分析统计。结果显示,GABA免疫阳性神经元、阳性纤维及终末在青年猫和老年猫小脑皮质各层均有分布。与青年猫相比,老年猫分子层、蒲肯野细胞层神经元和GABA免疫阳性神经元密度及其GABA免疫阳性反应强度均显著下降(P<0.01),颗粒层神经元密度和GABA免疫阳性强度也显著下降(P<0.01),但其GABA免疫阳性神经元密度无显著变化(P>0.05);蒲肯野细胞的胞体萎缩,阳性树突分枝减少。因此认为,衰老过程中猫小脑皮质GABA能神经元的丢失和GABA表达的下降,可能是老年个体运动协调、精确调速和运动学习等能力下降的重要原因之一。更多还原

【Abstract】 To investigate age-related changes of GABAergic neurons and GABA expression in the cerebellar cortex of young adult and old cats,we applied Nissl staining to show the structure and neurons in cerebellar cortex.Immunohistochemical method was used to show the GABA immunoreactive neurons.Under light microscope,the numbers of total neurons and GABAergic neurons in the molecular layer,Purkinje layer and granular layer were counted respectively,and the average gray value of GABA immunoreactive neurons was measured with Image-Pro Express Software.The GABA immunoreactive cells,the fibers and their terminals were distributed in each layer of cerebellar cortex in young adult and old cats.Compared to young cats,the number of the neurons and GABA immunoreactive neurons declined significantly in the molecular and Purkinje layers of old cats(P<0.01).However,no difference was observed in the number of GABA immunoreactive neurons in the granular layer of young and old cats(P>0.05).In addition,the intensity of GABA immunoreactivity was visibly weaker in old cats than in young adults(P<0.01).Purkinje cells in old cats showed much less GABA immunoreactive dendrites than in young cats.An evident loss of GABAergic neurons and decline of GABA expression during aging may result in the functional degeneration of motor coordination,accurate timing and motor learning in old individuals.更多还原