【摘要】 在细胞注射时,显微镜上CCD获取的只是注射针的二维信息,但光轴方向的深度信息丢失,这会导致细胞注射的失败,必须用其他方法获得。利用激光三角测量法对注射针以及细胞载玻片的深度信息进行测量,通过纳米平台的移动来标定激光入射角参数,对获取的数据进行图形化处理,确定线激光条纹图像间的偏移距离,从而获得标准物体高度与激光条纹图像偏移量的像素比值,该比值可以用来计算实际物体的高度。另外,利用三自由度机械手,对测量的探针离细胞载玻片的距离进行验证,获得激光三角测量法的误差值。实验结果表明,在细胞注射中,利用激光三角测量法获得注射针的深度信息是可行的。更多还原

【Abstract】 Only two dimensions information of injection needle could be captured by CCD in microscope image,and the lack of the depth information through the optical axis of microscope system would result in the failure of cell injection.A new way called laser triangulation to exract depth information of the injection needle and the slide glass was presented.The measuring principle was calibrating the projection angle of laser,θ was callibrated by the remotion of nano platform,then ascertain the displacement of laser stripe,S by image processing,The height of actual objects will be decided by S· tgθ.In addition,the error of laser triangulation is demonstrated to be about from 5 to 10 μm by calibration using a 3-DOF manipulators.The result indicates that it is feasible to measure the depth information of injection needle by laser triangulation with the required precision in cell injection.更多还原